IsoTag™ AAV REAGENT. FAST PURIFICATION AT YOUR FINGERTIPS.
WITH THE POWER OF IsoTag™ AAV REAGENT AT YOUR FINGERTIPS, YOU CAN PURIFY SAMPLES QUICKLY TO KEEP YOUR RESEARCH MOVING.
Get the results you need quickly with the IsoTag™ AAV Reagent Rapid Screening Kit.
The IsoTag™ AAV Rapid Screening Kit empowers you to use your existing lab setup to rapidly purify adeno-associated virus (AAV) harvest material in 2-3 hours. It leverages the novel IsoTag™ AAV reagent technology, which combines affinity and size-based purification into one straightforward protocol. This allows for fast and simple screening of multiple AAV Samples in parallel, facilitating confident decisions about therapeutic candidates.
Keep your research moving quickly with the IsoTag™ AAV Reagent Rapid Screening Kit.
The IsoTag™ AAV Rapid Screening Kit empowers you to use your existing lab setup to rapidly purify adeno-associated virus (AAV) harvest material in 2-3 hours. It leverages the novel IsoTag™ AAV reagent technology, which combines affinity and size-based purification into one straightforward protocol. This allows for fast and simple screening of multiple AAV Samples in parallel, facilitating confident decisions about therapeutic candidates.
One single reagent, compatible with AAV serotypes 1, 2, 3, 6, 8, 9, and PHP.B
Simple centrifugation-based protocol, with minimal hands-on time, that allows for screening multiple AAV samples in parallel
Purification can be completed in 2–3 hours
Flexibility to purify starting volumes ranging from 1 mL to 40 mL
No specialized equipment or extensive training required
Includes two elution buffers (low pH and neutral pH) to optimize capsid recovery
Purify with Ease. Get Results Fast. Make Decisions.
The IsoTag™ AAV Rapid Screening Kit offers scientists a low touchpoint solution for purifying multiple AAV samples in parallel in as little as 2 to 3 hours using a benchtop centrifuge. With no specialized equipment or extensive training required, any scientist can quickly and easily purify AAV samples.
The IsoTag™ AAV Rapid Screening Kit comes with 1 mL of IsoTag™ AAV reagent, and all the buffers you’ll need to purify and screen dozens of constructs, capsid variants, starting materials and process parameters using only a bench-top centrifuge.
Learn more about how the IsoTag™ AAV Rapid Screening Kit can help you quickly evaluate therapeutic candidates.
+ Ease of Use Regardless of Experience Level
The IsoTag™ AAV Rapid Screening Kit utilizes a simple, centrifugation-based protocol that shows consistent capture and elution performance regardless of user experience.
Novel IsoTag™ AAV Reagent Purification Technology
IsoTag™ AAV reagent is a self-scaffolding biopolymer with an affinity tag that selectively binds and separates AAV from cell contaminants via fully reversible phase separation.
Performance, Compatibility, and Versatility
IsoTag™ AAV reagent provides both the recovery and impurity clearance ideal for screening AAV harvest material samples. IsoTag™ AAV reagent showed out-of-the-box performance of over 75% capture of AAV 1, 2, 3, 6, 8, 9, and PHP.B.
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+ Out-of-the-box Capture and Elution for Multiple AAV Serotypes
As measured by qPCR
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+ Effective clearance of key contaminants and additives
Purification with IsoTag™ AAV reagent provides host cell protein and host cell DNA contaminant removal, as well as residual IsoTag™ AAV reagent clearance.
n=9, for AAV9 starting material volumes ranging from 1 mL to 40 mL. Host-cell protein levels assessed using Cygnus HEK293 HCP ELISA Kit, 3G. Host-cell DNA levels were assessed using Invitrogen Quant-iT™ PicoGreen™ dsDNA Assay Kit. Residual IsoTag™ AAV reagent levels were assessed using Cygnus IsoTag AAV- Residual Reagent ELISA.
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+ Consistent capture and elution at different starting volumes
IsoTag™ AAV reagent achieved consistent capture and elution for the same AAV9 starting material at 1 mL, 10 mL, and 40 mL starting volumes. This allows scientists the flexibility to purify starting volumes ranging from 1 mL to 40 mL with the one, single reagent and centrifugation-based protocol.
As measured by qPCR
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+ Purification of Clarified and Unclarified AAV Lysate
IsoTag™ AAV reagent has also shown comparable recovery and purification in 40 mL samples of both clarified and unclarified AAV lysate.
As measured by qPCR
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+ Ability to Concentrate 10X
IsoTag™ AAV reagent and its centrifugation-based protocol supports up to 10X concentration of elution volume, enabling workflow flexibility and higher AAV titers.
As measured by qPCR
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+ From Complex Lysate to Pure AAV — in hours
Get in touch to order your IsoTag™ AAV Rapid Screening Kit today.
IsoTag™ AAV Rapid Screening Kit: