Concentrate and purify AAV in under 4 hours.
The IsoTagTM AAV Rapid Screening Kit empowers you to use your existing lab setup to rapidly purify adeno-associated virus (AAV) harvest material in 2-3 hours.
It leverages our novel IsoTagTM AAV reagent technology, which combines affinity and size-based purification into one straightforward protocol. This allows for fast and simple screeningof multiple AAV samples in parallel, facilitating confident decisions about therapeutic candidates.
One single reagent, compatible with AAV serotypes 1, 2, 3, 6, 8, 9, and PHP.B
Simple centrifugation-based protocol, with minimal hands-on time, that allows for screening multiple AAV samples in parallel
Purification can be completed in 2–3 hours
Flexibility to purify starting volumes ranging from 1 mL to 40 mL
No specialized equipment or extensive training required
Includes two elution buffers (low pH and neutral pH) to optimize capsid recovery
Purify with Ease. Get Results Fast. Make Decisions.
The IsoTag™ AAV Rapid Screening Kit offers scientists a low touchpoint solution for purifying multiple AAV samples in parallel in as little as 2 to 3 hours using a benchtop centrifuge. With no specialized equipment or extensive training required, any scientist can quickly and easily purify AAV samples.
The IsoTag™ AAV Rapid Screening Kit comes with 1 mL of IsoTag™ AAV reagent, and all the buffers you’ll need to purify and screen dozens of constructs, capsid variants, starting materials and process parameters using only a bench-top centrifuge.
Learn more about how our IsoTag™ AAV Rapid Screening Kit can help you quickly evaluate therapeutic candidates.
+ Ease of Use Regardless of Experience Level
Novel IsoTag™ AAV Reagent Purification Technology
IsoTag™ AAV reagent is a self-scaffolding biopolymer with an affinity tag that selectively binds and separates AAV from cell contaminants via fully reversible phase separation.
Performance, Compatibility, and Versatility
IsoTag™ AAV reagent provides both the recovery and impurity clearance ideal for screening AAV harvest material samples. IsoTag™ AAV reagent showed out-of-the-box performance of > 75% capture of AAV 1, 2, 3, 6, 8, 9, and PHP.B.
+ Out-of-the-box Capture and Elution for Multiple AAV Serotypes
IsoTag™ AAV reagent provides both the recovery and impurity clearance ideal for screening AAV harvest material samples. IsoTag™ AAV reagent showed out-of-the-box performance of > 75% capture of AAV 1, 2, 3, 6, 8, 9, and PHP.B.
Contaminant removal from AAV9 across a range of sample volumes. Assessed across 9 replicates (n = 9) using sample volumes ranging from 1 to 40 mL.
+ Effective clearance of key contaminants and additives
+ Consistent capture and elution at different starting volumes
IsoTag™ AAV reagent achieved consistent capture and elution for the same AAV9 starting material at 1,10, and 40 mL starting volumes. This allows scientists the flexibility to purify starting volumes ranging from 1 mL to 40 mL with the one, single reagent and centrifugation-based protocol.
+ Scalability
ALPS-TFF scales linearly, increasing filter surface area with harvest volume
+ Yield
>90% capture and 75% elution of both total virus capsids
+ Quality
≥4 LRV of contaminating host cell proteins & DNA in harsh conditions
IsoTag™ AAV reagent provides both the recovery and impurity clearance ideal for screening AAV harvest material samples. IsoTag™ AAV reagent showed out-of-the-box performance of > 75% capture of AAV 1, 2, 3, 6, 8, 9, and PHP.B.
Performance,
Compatibility, and
Versatility
IsoTag™ AAV Reagent
This evaluation kit contains IsoTag™ AAV reagent and the buffers required to purify approximately 1.5 liters of clarified AAV harvest material in a TFF process.
Breaking free from Chromatography:
Transformative AAV Purification
Director of Advanced R&D, Michael Dzuricky, Ph.D., on how IsoTag™ AAV Reagent can achieve >90% AAV capture and 60-85% elution recoveries with our baseline protocol on the first run. View Webinar→
More on: IsoTag™ AAV
